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Nucleotide sequences of various forward (F) and reverse (R) primers used for RT-PCR.
| Target | Primers sequences (5′-3′) | Annealing Temp (°C) | Product size (bp) |
|---|---|---|---|
| IL-12p40 | F-GGCCCAGAGCAAGATGTGTCACCA | 65 | 144 |
| R-TCTCCAGGGGCATCCGGATACCAA | |||
| IL-10 | F-ACAGCTCACCACTGCTCTGT | 60 | 327 |
| R-AGTTCACATGCGCCTTGATG | |||
| GAPDH | F-GAAGGTGAAGGTCGGAGTC | 60 | 225 |
| R-GAAGATGGTGATGGGATTTC | |||
| TLR2 | F-GCCTACTGGGTGGAGAACCT | 60 | 340 |
| R-GGCCACTCCAGGTAGGTCTT | |||
| TLR4 | F-TGCAATGGATCAAGGACCAGAGGC | 62 | 449 |
| R-GTGCTGGGACACCACAACAATCACC |
