Fig. 2.

Detection of CXCR6 on PMN of patients with implant-associated osteomyelitis analysed by cytofluorometry. Expression of CXCR6 on PMN of patients (n = 10) with implant-associated osteomyelitis was measured on the day of surgery and on the 7th postoperative day and compared with healthy donors (n = 10) using cytofluorometry. Only a small amount of peripheral blood PMN expressed CXCR6, a representative example on the day of surgery is shown in (a). In contrast, nearly all PMN obtained from the infected site revealed distinct CXCR6 expression on the day of surgery; a representative example is shown in (b). After recovery, the peripheral blood PMN were negative for CXCR6 on the 7th postoperative day; a representative example is shown in (c); data of four patients are summarized in (d). In (e) the results of the CXCR6 expression from peripheral PMN of patients (n = 10) and controls (n = 10) as well as infiltrated PMN of patients (n = 10) are summarized as box plots (the box contains 50% of the values; the horizontal bar indicates the median, the dot the mean values; the differences between the mean values were evaluated by anova; n.d. = not different). The marker M1 was set for the isotype controls in all given experiments.