Fig. 4.

Chemotaxis of isolated PMN of healthy donors induced by recombinant CXCL16 or by supernatants of two pancreatic carcinoma cell lines. Recombinant human CXCL16 (rhCXCL16) induced chemotaxis of PMN (a), as did the supernatants of two pancreatic carcinoma cells lines (BxPC-3 and COLO-357), known to produce CXCL16 (b). When the chemokine production of these cell lines was inhibited by silencing via specific ADAM10 siRNA transfection with two different oligonucleotides (siRNA 1 and siRNA2), the chemotaxis was clearly reduced. The data are given as distance in µm covered by at least five PMN. Twenty filter areas were evaluated and the mean ± s.d. is shown. For comparison, on the very left, random migration as negative control and migration towards C5a as positive control are shown. (In (a) the P-values were calculated for the rhCXCL16-induced migration in comparison with random migration; in (b), in addition, differences between CXCL16-containing supernatants and supernatants of cells pre-treated with siRNA were calculated).