Fig. 3.

Effects of administration of various antigens on total cell and eosinophil number (primary axis) and protein content (secondary axis) in BALF. Saline, ovalbumin (OVA), Epicoccum extract (EE), Epi p 1 active protease (EAP) and Epi p 1 inactivated protease (EIAP) were administered to the mice in different groups. The protein concentration (shown as line plotted) was measured by Bradford assay. The total number of cells was counted using a haemocytometer. The eosinophils were counted on cell smears stained with Leishman's stain. Statistical difference in each parameter of EAP and EIAP sensitized mice is mentioned as P-value (at a confidence level of 0·05); *P < 0·05; **P < 0·01.