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. Author manuscript; available in PMC: 2008 Dec 31.
Published in final edited form as: J Mol Biol. 2008 Jun 17;381(3):707–717. doi: 10.1016/j.jmb.2008.06.021

Fig. 4.

Fig. 4

The GroEL–GroES–ADP crystal structure (PDB code 1AON; in ribbons) was docked into the 8-Å cryo-EM density map of the GroEL–Aacpn10–ADP complex using domain-as-rigid-body fitting. (a) Resolution assessment of 8 Å for the GroEL–Aacpn10–ADP cryo-EM structure with the use of a criterion of Fourier shell correlation at 0.5. (b) A central cross-section of the cis-ring along a 7-fold symmetry axis showing the overall good fitting of the cis-ring. (c) A cross-section through the cis-ring equatorial domain of GroEL showing the excellent correspondence of α-helices. (d) Fitting between the GroES structure (in yellow ribbon) and the dome-shaped cap of Aacpn10 showing that the roof loops fit well with the densities of Aacpn10 from the GroEL–Aacpn10–ADP complex. The ribbons from different domains of the crystal structure of GroEL–GroES–ADP in (b) are colored differently.