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. 2009 Jan;15(1):116–127. doi: 10.1261/rna.1080109

FIGURE 5.

FIGURE 5.

Identification of the 5′ and 3′ ends of var transcripts. (A) Schematic diagram showing the RNA circularization method for simultaneous mapping of 5′ and 3′ ends. (B) The DNA sequence of a portion of the intron from var gene PFD0005w. The 5′ ends of the sterile RNAs transcribed from this gene are marked with brackets. The ccc repeat motif is underlined. (C) The DNA sequence downstream from the coding region of PFD0005w. The stop codon of the open reading frame is underlined. The sterile RNAs transcribed from this gene ended within 20 bp of the stop codon as marked by the bracket. (D) The DNA sequence downstream from the coding region of var gene PFD1005c. The polyadenylation sites of the mRNA transcribed from this gene are marked with asterisks. The stop codon is underlined.