FRET distances in free SRP and the SRP–RNC complex. (A) Distances between position 344 in the M domain of Ffh and the indicated positions in the N domain (17, 25) and G domain (84, 152, 165, 201, 203). (B) Distances between the 3′ end of 4.5S RNA21–81 and the indicated positions in Ffh. (White bars) Free SRP; (black bars) SRP–RNC complex.