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Fractionation of tmRNA mutants. (A) TmRNAs were transcribed in vitro, radioactively labeled at the 3′ end by incubation with [α-32P]ATP and ATP/CTP tRNA nucleotidyl transferase, and fractionated by electrophoresis on a native 5% polyacrylamide gel. α and β denote tmRNA conformers. (B) Graphical representation of data derived from the polyacrylamide gel shown in A.
