FIGURE 3.

Overexpressed hnRNPs affect nonsense exon splicing. Xpress-tagged hnRNP expression plasmids were cotransfected with the AAG splicing reporter. Top panel, agarose gel of RT-PCR products. Middle panel, Western blot using antibodies against the Xpress tag and actin. Lower panel, histogram indicating levels of exon inclusion from three biological repeats. Error bars indicate standard deviation. Single dots indicate P < 0.05, double dots P < 0.01 in pairwise t-test comparing control to overexpression lanes. HnRNPs F, H, and PTB repressed the nonsense exon while hnRNP A1 promoted inclusion.