FIGURE 4.

hnRNP H and F repression is mediated by ESSs 1 and 2. The “WT” AAG construct and the 13 derived ESE/ESS mutants were transfected into HeLa cells alone (top panels) or cotransfected with hnRNP F (middle panels) or hnRNP H (lower panels) expression vectors. In each set of panels, RT-PCR agarose gels are on top, and Western blots are below. The histogram below summarizes data from three biological replicates. Black bars, control, gray bars, hnRNP F overexpression, white bars, hnRNP H overexpression. Single dots indicate P < 0.05, double dots P < 0.01 in pairwise t-test comparing control to overexpression lanes. The repressive effects of hnRNP F and H were completely abrogated by mutations 1 and 2, which affected a G-rich ESS.