Western blot analysis for neuronal markers (PGP 9.5, HuD), transporter SERT, α-smooth muscle actin (α-SMA), Glial Fibrillary Acidic Protein (GFAP) and β-actin as a loading control. Neuronal markers were present in both IM-FEN and IM-PEN and there was very minimal expression of α-SMA and GFAP at 39°C. Results are mean + S.E., n=3, ** = p<0.01, *** = p<0.001.