Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2009 May 1.

Published in final edited form as: Gastroenterology. 2008 Feb 14;134(5):1424–1435. doi: 10.1053/j.gastro.2008.02.018

(a) Representative images of fetal and post natal enteric neurons cultured for 7 days at 39°C and stained with PGP9.5 and DAPI. (b) Higher power view of a (IM-FEN and IM-PEN) neuron with neurites stained with PGP9.5 is shown. (c) Neuronal network stained with PGP9.5 (red) or Peripherin (red) and DAPI. (d) Histogram shows the relative percentages of PGP9.5 positive cells at 33°C and 39°C. (e) Using IM-FEN cells double labeling was performed with peripherin (red) in conjunction with other stated markers (arrow, green). Nuclear stain is shown with DAPI staining. Negative controls with isotopic antibody to peripherin and peripherin staining of epithelial cell line, stellate cells and glial cells are shown. Positive controls for α-SMA (activated stellate cells) and for S-100β (cortical glial cells) are shown. (f) Histogram shows the stated cell markers expressed as a percentage of DAPI⁺ cells. Results are mean + S.E., n=4, *** = p<0.001. Scale bar: 20μm.

(a) Representative images of fetal and post natal enteric neurons cultured for 7 days at 39°C and stained with PGP9.5 and DAPI. (b) Higher power view of a (IM-FEN and IM-PEN) neuron with neurites stained with PGP9.5 is shown. (c) Neuronal network stained with PGP9.5 (red) or Peripherin (red) and DAPI. (d) Histogram shows the relative percentages of PGP9.5 positive cells at 33°C and 39°C. (e) Using IM-FEN cells double labeling was performed with peripherin (red) in conjunction with other stated markers (arrow, green). Nuclear stain is shown with DAPI staining. Negative controls with isotopic antibody to peripherin and peripherin staining of epithelial cell line, stellate cells and glial cells are shown. Positive controls for α-SMA (activated stellate cells) and for S-100β (cortical glial cells) are shown. (f) Histogram shows the stated cell markers expressed as a percentage of DAPI⁺ cells. Results are mean + S.E., n=4, *** = p<0.001. Scale bar: 20μm.