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. Author manuscript; available in PMC: 2009 Oct 15.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Sep 12;874(1-2):84–88. doi: 10.1016/j.jchromb.2008.09.007

Table 1.

Back-calculated concentrations from calibration curves over the concentration range of 0.2 to 500 ng/mL.a

Nominal Concentration (ng/mL)
0.2 1.0 5.0 10.0 50.0 100 500
Accuracy
(%)		 102.8 98.4 98.4 100.2 99.4 101.0 99.9
Concentration 0.21 ± 0.98 ± 4.9 ± 10.0 ± 49.7 ± 101.0 ± 499.4 ±
(ng/mL) 0.031 0.077 0.46 0.42 2.1 6.4 28.5
Precision (%)
Within-run 12.4 9.9 2.7 3.8 4.9 8.0 7.5
Between-run 8.9 b 9.8 1.9 b b b
No. of
samples		 8 8 8 8 8 8 8
a

Performed in duplicate on 4 separate days.

b

No significant variation was observed as a result of performing the assay in different runs.