Figure 4.

Expression analysis of npcRNAs. (A) Accumulation of npc531 transcripts in dcl1 mutants. Real-time RT-PCR expression analysis of npc531 in wild-type and dcl1-9 inflorescences (A) was normalized with the ACT2 (AT3G18780) gene, and values for wild type were arbitrarily fixed to 1. On the right, predicted pairing between npc531 and miR319a. (B) Regulation of npc43, npc60, npc72, and npc536 expression. Northern analysis was performed on the indicated npcRNAs under different stress conditions (plants grown in phosphate starvation or under salt stress).