Skip to main content
. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Immunity. 2008 Aug 14;29(3):391–403. doi: 10.1016/j.immuni.2008.07.011

Figure 6. Cytokine profile of PLZF Transgenic CD4 T cells.

Figure 6

(A) Intracellular flow cytometry with anti-PLZF mAb D-9 of thymic PLZF-transgenic (line 1797) CD4 SP cells compared with wt CD4 SP and with wt NKT stage 1 + 2 cells thymocytes (Tetr+NK1.1−), as indicated.

(B) Decreased IL-2 secretion by PLZF-transgenic CD4 T cells. CD4 cells were MACS-enriched from spleens of PLZF-transgenic and littermate and stimulated with anti-CD3 + human IL-2 for two days. Values in pg/ml for 2 pairs of wt and Tg littermates, as indicated.

(C) Intracellular flow cytometry for IL-4 and IFN-γ in littermate and PLZF transgenic mice. Splenocytes were prepared and stimulated as in A, with the addition of brefeldin A for the last six hours of stimulation. Three pairs of wt and Tg littermates were analyzed. The percentages of dual IL-4/ IFN-γ producers were 24.5, 17.3, 26.1 in transgenics vs 3.97, 4.92, 7.34 in wt.