Table 2.
Labeling of sub-retinal debris
| Label | Source | Dilution | SRD |
|---|---|---|---|
| SRD: sub-retinal debris, UC: unesterified cholesterol, EC: esterified cholesterol, present (+), minimal present (+/−), absent (−). | |||
| Antibodies | |||
| GFAP | Dako | 1:100 | − |
| CRALBP | Gift from John C Saari | 1:2500 | − |
| Rhodopsin (R4D2) | Gift from Robert Molday | 1:50 | − |
| Red-green opsin | Gift from Jeremy Nathans | 1:50 | − |
| ApoE | Calbiochem | 1:100 | + |
| ApoB | Polysciences, Inc. | 1:100 | +/− |
| ApoA-l | Polysciences, Inc. | 1:100 | +/− |
| Complement Factor H | Quidel | 1:200 | + |
| Vitronectin | Santa Cruz | 1:200 | + |
| Lectins | |||
| PNA | EY Laboratories | 1:1000 | − |
| PNA | Vector Laboratories | 1:500 | − |
| WGA | EY Laboratories | 1:200 | − |
| Lipid Stains | |||
| Filipin | Sigma | 500 μg/ml | + UC |
| +/− EC | |||