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. Author manuscript; available in PMC: 2009 Aug 1.

Published in final edited form as: J Mol Cell Cardiol. 2008 May 20;45(2):270–280. doi: 10.1016/j.yjmcc.2008.05.006

A, Uninfected neonatal cardiomyocytes (control) or myocytes infected with Ad.β-Gal or Ad.Retn were incubated for 48 hours before staining with Alexa 594-conjugated phallodin. Endothelin-1 (ET-1), a hypertrophic agonist used as a positive control, induced highly organized sarcomere structures. Likewise, Ad.Retn-infected myocytes exhibit a large number of cells with highly organized sarcomeres compared with control or Ad.β-Gal-infected cells. At least 200 cells per condition were scored for the presence of highly organized sarcomeres. B, Cell surface areas of more than 100 individualized cells per condition from 3 independent experiments were measured using Image J software. ^¤P<0.01 ET vs control; *P<0.01 Ad.Retn vs Ad.β-Gal. The mean values± SD are shown.

A, Uninfected neonatal cardiomyocytes (control) or myocytes infected with Ad.β-Gal or Ad.Retn were incubated for 48 hours before staining with Alexa 594-conjugated phallodin. Endothelin-1 (ET-1), a hypertrophic agonist used as a positive control, induced highly organized sarcomere structures. Likewise, Ad.Retn-infected myocytes exhibit a large number of cells with highly organized sarcomeres compared with control or Ad.β-Gal-infected cells. At least 200 cells per condition were scored for the presence of highly organized sarcomeres. B, Cell surface areas of more than 100 individualized cells per condition from 3 independent experiments were measured using Image J software. ^¤P<0.01 ET vs control; *P<0.01 Ad.Retn vs Ad.β-Gal. The mean values± SD are shown.