Figure 6.
MIG-10::YFP localizes ventrally in a netrin-dependent manner.(a–g) Lateral views of L3 stage larvae expressing unc-86::YFP::mig-10. The HSN cell body fills most of each panel. Traces are line-scan intensity plots (in arbitrary units) of the YFP signal around the periphery of the cell shown, beginning at the arrow and moving clockwise. IR, intensity ratio. In b, mig-10 mutant rescued by the mig-10::YFP transgene. (h) Average intensity ratios of MIG-10::YFP signals. Ratio was calculated as the intensity of the ventral half of the perimeter divided by the dorsal half (n = 9–14 cells for each genotype). The random distribution of myr-GFP in HSN is included as a control. Error bars indicate s.e.m. By Bonferroni t-tests (P < 0.05), wild type, mig-10 and unc-34 were indistinguishable; unc-6, unc-40and daf-18 were highly defective; and age-1 was intermediate. In all pictures, ventral is down and anterior is to the left. Scale bar, 5 μm.
