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. Author manuscript; available in PMC: 2009 Aug 22.
Published in final edited form as: Cell. 2008 Aug 22;134(4):599–609. doi: 10.1016/j.cell.2008.06.033

Figure 3. Test for CES function in a luciferase reporter assay.

Figure 3

(A) Experimental design, and structure of the constructs used for a dual Firefly/Renilla luciferase reporter assay. S2 cells co-transfected with the firefly luciferase construct containing each 1.5 kb CES fragment and the control Renilla luciferase plasmid were treated with dsRNA against either GFP (control) or MSL2 (experimental). (B) Examples of Firefly/Renilla luciferase activity conferred by nine chromatin entry sites normalized to the value obtained after msl2 RNAi. CES18D11 is a rare example of a CES that failed to show MSL2-dependent upregulation in this assay.