Table 1.

Primer sequences used for qPCR

Name	Product length	Primer efficiency	Primer sequence	Base pair no.	Accession no.
KCNQ5	225 bp	100	CTC ACC CCA CCA CTT AAA	1623–1640	NM_019842
			CTC TCG GCT CTT CTT ATC TG	1847–1828
SUR1	145 bp	91	TCG CCG GGA CAG AAG ATT GG	911–930	AF183921
			GGG TGT GCA GTG GCA GTT TG	1055–1036
GEC-1	234 bp	100	GCG CGA GTC TTC ATC AAG GG	244–263	AF012920.2
			TGA GGT CAG AGG GCA CAA GG	477–458
ERα	56	97	CTG CGC AGT GTG CAA TGA C	19–37	DQ218311
			TCA CAG GAC CAG ACC CCA TAA	75–55
ERβ	58	99	AGA ACC GGC GGA AAA GCT	96–113	DQ218312
			CAT TCC TAC TCC ATA GCA CTT TCG	154–131
Cav3.1	133 bp	96	CTG GAG CTG GAG ATG AAG AC	162–181	DQ992353.1
			AGT GGG CTG CCT TGT ATG	294–277
CaM-1	176 bp	92	TCT GCA CAT CAT CCT GAG	164–181	NM_009790
			CAT GCA GCT TGA CGA TTG	339–322
AKAP11	177 bp	94	GTC CAA TGA TGG CAA GTC AG	217–236	NM_144490
			CGT GCA ATC CAT CTA CGC AG	393–374
PKAα1	162 bp	95	GAG CAG CCA CTG TCA AAG C	827–845	NM_212471
			CGT TCC CAC TTG TCC AGA G	988–970
PI3K p85α	190 bp	93	GCC AGA CCT CAT TCA GCT AAG	1701–1721	NM_174575
			GTT GCT GCT GCC AAC ATT C	1890–1872
GLRβ	246 bp	98	CAT GCC CTT TGG ACT TGA C	621–639	NM_000824
			TCC TCA GGG TGA AGA TGA C	866–848
CaMKIIα	101 bp	93	GAA GAC ACC AAA GTG CGG AAA C	1151–1172	BC040457
			TCG CAC ATC TTC GTG TAG GAC	1251–1231
PITPβ	114 bp	93	GTTG TGC CTA TAA GCT GGT GAC	602–622	BC031427
			AGC TGG CGA TGG AAG TTT G	715–697
TH	223 bp	93	TCC ACG TTA TAC TGG TTC AC	64–83	DQ992354
			TTG CAT CAC TGA AGC TCT C	286–268
GAPDH	212 bp	100	CAT CCA CTG GTG CTG CCA	123–142	U51572
			GTC CTC GGT GTA GCC CAA G	334–315
NPY	91	94	CCC GAG ACA CTG ATT TCA GAT CT	294–316	NM_000905
	Probe		CCC ATC ACC ACA TAG AAG GAT CTT	384–361
			AAA GCA CAG AAA ATG TCC CCA GAA CTA GGC	328–357
POMC	63	96	CGC TGG TGA CGC TGT TCA	713–730	S78260.1
	Probe		CGC CTC ACT GGC CCT TCT	775–758
			AAC GCC ATC GTC AAG A	733–748
β -Actin	60	100	AAG CCC AGA GCA AAA GAG GTA TC	177–199	AF508792
	Probe		TAA CGA TGC CGT GCT CAA TG	236–217
			TGACCCTGAAATACC	201–215

The sense primer is listed first with the antisense primer below.