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. 2008 Aug 28;149(12):6006–6017. doi: 10.1210/en.2008-0274

Figure 4.

Figure 4

Expression of gephyrin and CASK but not PSD-95 in islets and β-cell lines. Western blot analysis was performed on islet and β-cell line extracts using antibodies to gephyrin, PSD-95, and CASK. Brain (Br) lysates were used as positive controls. As expected, gephyrin, PSD-95, and CASK were detected in human (H), mouse (M), and rat (R) brain near their expected molecular masses of 93, 95, and 120 kDa, respectively. Gephyrin was also detected in human islets, rat islets, INS-1 cells, NIT-1 cells, and αTC-6 cells (with the latter two extracts yielding a weaker band). α-TC6 is a partially dedifferentiated islet α-cell line. PSD-95 was not detected in human islets, rat islets, or any of the islet cell lines tested. CASK was detected in human islets, rat islets, and all islet cell lines. α, αtc-6 cell lysate; Isl, islet tissue lysate; Ins, INS-1 cell lysate; Nit, NIT-1 cell lysate.