Figure 4.
KAP3 mRNA is expressed in glutamatergic but not GABAergic neurons of the brain. The in situ hybridization reaction was performed on brain sections derived from three 28- to 30-d-old female rats. The VMH of the hypothalamus and the CTX are used to illustrate this cellular localization. A, The VMH is rich in cells containing KAP3 mRNA (identified with a digoxigenin-labeled cRNA probe, brown staining) but lacks GAD67 mRNA-positive cells (identified with a [35S]UTP-labeled probe, white grains). B, VMH cells contain an abundance of both VGLUT2 and KAP3 mRNA transcripts. C, High-magnification view showing that GAD67 mRNA-containing cells (examples denoted by arrows) in the CTX are different from cells expressing KAP3 mRNA. D, In the VMH, all cells containing VGLUT2 mRNA (white grains) are also KAP3 mRNA positive (brown staining). Cells that are negative for VGLUT2 mRNA also lack KAP3 mRNA (asterisks). Bars, 200 μm (A and B) and 20 μm (C).