Figure 6.

Stimulatory effect of EB on the MBH and ME content of KAP3 protein and suppressive effect of in vivo inhibition of KAP3 synthesis via administration of an AS ODN on the content of KAP3 in the MBH and ME of OVX rats and OVX rats treated with EB as determined by Western blot analysis. A, Representative Western blots; B, quantification of the changes observed in A. Band densities were normalized using the densities of β-tubulin (β-tub) as the normalizing unit and were calculated as percentage of the values observed in samples from OVX+V (vehicle, sesame oil) animals treated with SCR ODN. Each value represents the mean of five to seven independent observations, and vertical lines are sem. Notice that treatment of OVX animals with EB induced a significant increase in KAP3 contents both in the ME and MBH. AU, Arbitrary units. *, P < 0.05; **, P < 0.01 vs. SCR ODN (SCR)-injected controls.