Figure 7.

Absence of an estrogen effect on the MBH and ME content of VGLUT2 and Syp proteins and suppressive effect of in vivo inhibition of KAP3 synthesis via administration of an AS ODN on the content of VGLUT2 and Syp in the ME of OVX rats and OVX rats treated with EB as determined by Western blot analysis. A, Representative Western blots showing that inhibition of KAP3 synthesis decreases VGLUT2 protein content in the MBH and ME. β-Tubulin (β-tub) was used as an internal control. B, Quantification of the changes shown in A. C, Representative Western blots showing that inhibition of KAP3 synthesis decreases the Syp content in the ME but not the MBH. D, Quantification of the changes illustrated in C. Band densities were normalized using the densities of β-tubulin as the normalizing unit and were calculated as percentage of the values observed in samples from OVX+V animals treated with SCR ODN. B and D, Each bar represents the mean of six Western blots. Vertical lines are sem. AU, Arbitrary units. *, P < 0.05; **, P < 0.01 vs. SCR ODN (SCR)-injected controls.