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. 2008 Aug 7;149(12):6065–6075. doi: 10.1210/en.2008-0687

Figure 1.

Figure 1

A medium induces rapid adipogenesis of C3H10T1/2 cells. C3H10T1/2 cells were seeded in six-well BioFlex Collagen-I coated plates (100,000 cells per well) in MEMα and cultured in M or A medium for 3 or 5 d. A, Cytoplasmic triglyceride droplets appeared at 3 d under adipogenic conditions, shown at ×40. B, Real-time RT-PCR was performed for PPARγ and adiponectin. mRNA is represented as a percent mRNA level measured in MEMα at d 0 (100%). *, P < 0.001 difference between M and A media on the same day. C, Immunoblot for PPARγ and adiponectin and actin: lanes refer to cells grown in M or A conditions for 3 or 5 d. CTL, Control.