Figure 4.

Inhibition of GSK3β mimics effects of strain. Inhibitors of GSK3 were added to cells cultured in A medium for 3 d. A, Cytoplasmic triglyceride droplets are shown with bright-field microscopy and compared with effect of strain (magnification, ×40). B, Real-time RT-PCR for mRNA species as noted showed effects of 3 d treatment with LiCl (10 mm) or the GSK3 inhibitor SB415286 (SB41, 20 μm). C, Western blotting of total cellular proteins showed that inhibition of GSK3 prevented events associated with adipogenesis. Act, Active; APN, adiponectin; cat, catenin; CTL, control.