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. 2008 Aug 7;149(12):6065–6075. doi: 10.1210/en.2008-0687

Figure 5.

Figure 5

Strain inactivates GSK3β. Cultures in M medium without strain (M) or A medium ± mechanical strain are shown after 3 (left) or 5 d (right). A, Total cellular proteins were blotted with antibodies for phospho-Akt (Ser473, pAkt), phospho-GSK3β (Ser9, pGSK3β), active (act) β-catenin (cat), PPARγ, adiponectin (APN), and actin (loading control). M+S, +strain; likewise A+S. B, On d 3, cells in either M or A medium were strained for 1, 15, and 30 min, or 6 h, and protein was extracted immediately for Western analysis. Below, Graphs show average densitometry from three experiments: both pAkt and pGSK3β increased with strain at 15 and 30 min in M medium but not until 6 h in A medium (P < 0.05).