Figure 6.

Mechanical inhibition of adipogenesis requires application early and repetitively. A, Strain was begun on the first day of adipogenic stimulus, and either dosed for 1 (S1) or 3 d (S3); mRNA for PPARγ and adiponectin (APN) was analyzed after 3 d in the A medium. Only the S3 condition inhibited adipocytic mRNA (P < 0.05). B, PPARγ and adiponectin protein from 3-d cultures in A medium: S1, Cells strained first day only; S3, strain each day of culture. C, Strain application was delayed for 2 d (A2+S3) or applied daily (S5) and protein analyzed at 5 d. D, Western blotting of total cellular protein of cells cultured in A medium ± strain for 5 d shows expected strain effects (left side of blot); culture for a further 3 d without strain shows equilibration between proteins studied in the two culture conditions (right side of blot). Act, Active; cat, catenin; CTL, control.