Table 1. Primer and probe sequences for OPA1, Bax, Bcl-2, and GAPDH for Taqman quantitative PCRa.
| Gene (GenBank) | Type | Sequences (5′–3′) |
|---|---|---|
|
OPA1 rat (NM_133585) |
Forward |
CAGCTGGCAGAAGATCTCAAG |
| |
Reverse |
CATGAGCAGGATTTTGACACC |
| |
Probe |
Universal Probe Library probe #2 |
| |
|
Cat. # 04684982001 |
|
Bax rat (NM_017059) |
Forward |
GTGAGCGGCTGCTTGTCT |
| |
Reverse |
GTGAGCGGCTGC-TTGTCT |
| |
Probe |
Universal Probe Library probe #83 |
| |
|
Cat. # 04689062001 |
|
Bcl-2 rat (NM_016993) |
Forward |
GGGATGCCTTTGTGGAACT |
| |
Reverse |
CTGAGCAGCGTCTTCAGAGA |
| |
Probe |
Universal Probe Library probe #2 |
| |
|
Cat. # 04684982001 |
|
GAPDH rat (X02231) |
Forward |
GAACATCATCCCTGCATCCA |
| |
Reverse |
CCAGTGAGCTTCCCGTTCA |
| Probe | CTTGCCCACAGCCTTGGCAGC |
aTaqman probe containing 5′ reporter FAM and 3′ quencher BHQ-1 dyes.