Figure 5.

Decreasing the antioxidant capacity of MDSCs decreases the ability of MDSCs to regenerate dystrophin-positive myofibers. MDSCs treated and untreated with 50 μM of DEM and myoblasts were injected into the skeletal muscle of Mdx mice that were sacrificed at 2 wk, and the regeneration index (RI) was measured. (A–C) Representative images of the dystrophin-positive engraftments of MDSCs, skeletal muscles treated with MDSCs, and myoblasts, respectively, are shown. (B) Bar, 50 μm. (D) MDSCs treated with 50 μM of DEM had regeneration indices similar to that of myoblasts and statistically significant lower regeneration indices than untreated MDSCs. ANOVA; *p ≤ 0.05; n = 8 in each group; mean values ± SEM.