Skip to main content
. 2009 Jan 1;20(1):306–318. doi: 10.1091/mbc.E08-06-0587

Figure 7.

Figure 7.

HAX-1 overexpression down-regulates endogenous SERCA2 protein levels. (A) Western blot analysis of HEK 293 cells transfected with the indicated constructs determined a significant reduction in the SERCA2 protein levels as the result of HAX-1 overexpression. HEK 293 cell lysates were separated by SDS-PAGE and incubated with SERCA2, calreticulin (CALRT), VDAC1, or GAPDH antibodies. A representative Western blot from a single experiment is shown. (B) Quantitative analysis of SERCA2, calreticulin (CALRT), VDAC1, or GAPDH protein levels from three independent experiments determined a statistically significant down-regulation of SERCA2 protein levels in HAX-1 transfected cells, compared with controls. Data are presented as means ± SE (n = 3; t test, *p < 0.05). (C) RT-PCR analysis of HEK 293 cells transfected with the indicated constructs did not identify any changes at SERCA2 mRNA levels.