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. 2009 Jan 1;20(1):419–427. doi: 10.1091/mbc.E08-08-0792

Figure 4.

Figure 4.

PCAF acetylates β-catenin in vitro and in vivo. (A) PCAF acetylated β-catenin in vitro. The acetylation of GST-β-catenin by PCAF was measured by in vitro acetylation assay and finally detected by Western blot. (B) PCAF acetylated exogenous β-catenin. After transfected with T41A-β-catenin-FLAG and PCAF-FLAG or ΔHAT2-PCAF-FLAG for 40 h, HEK293T cells were harvested for immunoprecipitation. The same membrane was stripped and reblotted with anti-β-catenin antibody. (C) PCAF acetylated endogenous β-catenin. After transfected with PCAF-FLAG or ΔHAT2-PCAF-FLAG for 40 h, HEK293T cells were harvested for immunoprecipitation with anti-β-catenin antibody. Western blots were performed with the indicated antibodies. The immunoprecipitated protein level was balanced to detect its acetylation level.