ADMA and L-NAME but not SDMA increase endothelial permeability in vitro. (A, C, E, and G) Distribution of F-actin and VE-cadherin (B, D, F, and H) in PAECs treated with SDMA (100 μmol/l), ADMA (100 μmol/l), or L-NAME (1 mmol/l) for 24 h. Bar, 10 μm. (I) shows changes in endothelial permeability in PAECs treated with the inhibitors. ** p < 0.01, comparison with untreated controls, n = 5.