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. 2009 Jan 1;20(1):33–42. doi: 10.1091/mbc.E08-04-0395

Figure 4.

Figure 4.

Rac1 but not RhoA or Cdc42 mediates the effects of ADMA on endothelial actin cytoskeleton (A and D), VE-cadherin (B and E), and endothelial permeability (G). (C and F) are corresponding images showing expression marker, c-myc. Cells in A–C were overexpressing N19RhoA in the presence of ADMA, whereas cells in D–F were overexpressing V12Rac1 in the presence of ADMA. Bar, 10 μm. (H) The graph shows the effects of recombinant DDAH on Rac1 activity; (I) the graph shows the effects of SNAP, 8Br-cGMP, and Rp-8-pCPT-cGMPS on Rac1 activity in untreated and ADMA-treated PAECs. Representative examples of Western blots are shown below the graphs. * p < 0.05; ** p < 0.01, comparison with untreated controls. # p < 0.05; ## p < 0.01, comparison with ADMA-treated cells. (G) n = 6; (H and I) n = 4. Adenoviral gene transfer was used to express recombinant Rho GTPases AdV12Rac1, AdN19RhoA, and AdN17Cdc42 as well as AdDDAHI, AdDDAHII, and inactive mutants AdDDAHIM, AdDDAHIIM.