Figure 1.

Genetic interaction profiles of yeast condensin. (A) Synthetic genetic interactions between the hypomorphic condensin allele ycs4-2::NAT (YBL186-16b) and indicated yeast deletion strains (xxxΔ::KAN). The systematic generation of double mutant combinations was performed in parallel and in quadruplicate using systematic genetic analysis technology (Tong et al., 2001) using the yeast knock out collection. Synthetic growth defects were initially determined by comparing growth of single versus double mutants at 23°C, the permissive temperature for all the condensin mutants. Interactions were individually confirmed by tetrad analysis and/or random spore analysis plus PCR to confirm the identity of the deleted locus and its linkage with the KAN marker. Filled and open circles denote synthetic lethal and sick interactions, respectively. (B) Verification by random spore analysis. Sporulated cultures (genotypes as indicated) were digested to release spores, and fivefold serial dilutions were plated on media that selects for MATa haploid single or double mutants. Genetic interactions were observable as a decrease in viable double mutant progeny (haploid selection plus clonNAT and G418) versus single mutant progeny. (C) Comparison of genetic interaction profiles between condensin mutants.