Figure 3.
Nucleolar enrichment of condensin in tof2Δ cells. (A) Chromatin spreads of the condensin subunit Smc4-HA in the presence (YBL194-1) or absence of Tof2 (YBL593-1) or Fob1 (YBL594-1) were performed in metaphase- versus anaphase-arrested cells. Micrographs show the localization of condensin predominantly in a subnuclear, DAPI-poor region characteristic of the rDNA. (B) Quantitation of the data shown in A. At least 100 nuclei is scored per sample.