Figure 9.

Colocalization of minireceptors with endosomal markers EEA1 and SNX17. MDCK cells were microinjected with the plasmids encoding for RAP, SNX17-myc, and either the mLRP4wt or N₂₆A minireceptors. Cells were processed for immunofluorescence to detect the HA-tagged minireceptor (green), the early endosome marker EEA1 (red), and the myc-tagged SNX17 (blue). In the amplified merged images, colocalization of the wild-type minireceptor with EEA1 in endosome-like structures was clearly visible; some of these structures also contain SNX17 (white dots). The N₂₆A minireceptors practically did not colocalize with SNX17 and exhibited an increased colocalization with EEA1 (amplified merge image, yellow dots). Scale bars, 10 μm.