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. 2009 Jan 1;20(1):452–463. doi: 10.1091/mbc.E07-11-1197

Figure 5.

Figure 5.

RhoE siRNA increases the pool of undifferentiated keratinocytes and promoted proliferation. (A) Cells treated with control or RhoE siRNA were induced to differentiate for 3 h and assessed for their FSC and SSC properties by fluorescence-activated cell sorting sorting. The gate used for separation of undifferentiated cells is shown. (B) Quantification of the data shown in A. (C) Keratinocytes treated with siRNA control oligos or RhoE-specific oligos were induced to differentiate, labeled with BrdU and analyzed by FACS (BrdU +ve). (D and E) Keratinocytes treated with nontargeting control, RhoE (D) or ROCK1 (E) siRNA oligos were induced to differentiate for up to 24 h, and lysates probed for phosphorylated Erk1/2, and actin. Error bars represent SE. N = 3; **p < 0.02 and *p < 0.03.