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. 2009 Jan 1;20(1):10–20. doi: 10.1091/mbc.E08-03-0324

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© 2008 by The American Society for Cell Biology

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Figure 8. — Phosphorylation of S821 is important for kinetochore recruitment of Mps1 but not for centrosome localization. (A) Kinetochore targeting of YFP-Mps1 and YFP-Mps1^S821→A in nocodazole-arrested mitotic cells. Cells were treated and analyzed as described in Figure 1B. (B) Centrosome localization of YFP-Mps1 and YFP-Mps1^S821→A in interphase cells. (C) Quantitation of fluorescent density of YFP-Mps1 and YFP-Mps1^S821→A on the kinetochores of prometaphase cells. The differences between YFP-Mps1 and YFP-Mps1^S821→A are statistically significant (p < 0.001).