Figure 7.

Fpr3 possesses chaperone activity and can suppress the heat sensitivity of Δynm3. (A) A single copy plasmid carrying FPR3 under the control of the MET25 promoter partially rescued the heat sensitivity of a Δynm3 strain under inducing conditions. (B) Fpr3, in substoichiometric amounts (0.03 μM) completely suppressed light scattering due to thermal aggregation of CS. (C) Equimolar amounts of Fpr3 dramatically reduced the rate of thermal inactivation of CS by ∼18-fold. (D) Deletion of FPR3 alone did not lead to heat sensitivity and double deletion of YNM3 and FPR3 was not synthetic lethal.