Table 1.
Effect of various treatments on CT
| Experimental manipulation | n | Cmax (%) | Cmin (%) | C60 (%) |
|---|---|---|---|---|
| Force magnitude | ||||
| 1 cm H2O | 3 | 101.6 ± 10.9 | 29.6 ± 1.2 | 38.6 ± 6.9 |
| 2 cm H2Oa | 3 | 99.3 ± 9.6 | 30.0 ± 3.6 | 35.0 ± 4.9 |
| 4 cm H2O | 3 | 115.0 ± 13.6 | 20.0 ± 2.3 | 25.3 ± 2.0 |
| 8 cm H2O | 3 | 107.6 ± 14.4 | 18.3 ± 0.8 | 26.6 ± 3.1 |
| 16 cm H2O | 3 | 89.6 ± 2.9 | 15.3 ± 0.8 | 18.6 ± 0.3 |
| Fill speed | ||||
| 2 cm H2O/2 mma | 3 | 99.3 ± 9.6 | 30.0 ± 3.6 | 35.0 ± 4.9 |
| 2 cm H2O/18 g | 4 | 93.0 ± 4.8 | 38.0 ± 1.6 | 51.7 ± 0.7 |
| 2 cm H2O/20 gb | 7 | 106.2 ± 9.6 | 35.0 ± 3.7 | 51.5 ± 5.6 |
| 2 cm H2O/22 g | 3 | 95.7 ± 13.5 | 25.7 ± 5.5 | 50.0 ± 7.0 |
| 2 cm H2O/25 g | 3 | 88.3 ± 7.6 | 36.0 ± 5.6 | 50.3 ± 6.1 |
| Temp or BFA | ||||
| Controlb | 7 | 106.2 ± 9.6 | 35.0 ± 3.7 | 51.5 ± 5.6 |
| 4°C | 5 | 100.0 ± 4.6 | 36.5 ± 4.6 | 20.0 ± 4.4* |
| BFA | 3 | 104.6 ± 8.3 | 24.6 ± 0.3 | 28.0 ± 0.5 |
| Cytoskeleton disruptors | ||||
| Cytochalasin D | 4 | 38.2 ± 4.1* | 21.2 ± 4.8 | 43.7 ± 4.0 |
| Latrunculin A | 3 | 71.0 ± 4.5 | 33.3 ± 5.2 | 58.0 ± 6.6 |
| Colchicine | 3 | 99.6 ± 10.4 | 36.0 ± 6.8 | 46.3 ± 2.3 |
| Thioglycolate | 4 | 53.2 ± 5.2* | 30.5 ± 5.2 | 51.0 ± 8.0 |
| Okadaic acid | 4 | 43.0 ± 7.0* | 29.0 ± 3.8 | 36.7 ± 4.9 |
| Channel blockers | ||||
| Gd3Cl, mucosal | 4 | 47.0 ± 3.3* | 28.2 ± 3.7 | 45.0 ± 8.9 |
| La3Cl, mucosal | 3 | 65.3 ± 10.6* | 25.6 ± 2.9 | 40.0 ± 3.7 |
| 100 μM amiloride, mucosal | 3 | 44.3 ± 2.1* | 30.3 ± 3.3 | 45.6 ± 3.8 |
| 1 μM amiloride, mucosal | 4 | 42.2 ± 10.2* | 21.0 ± 7.1 | 33.5 ± 8.9 |
| Benzamil, mucosal | 3 | 35.6 ± 12.3* | 20.3 ± 4.6 | 39.0 ± 5.1 |
| Gd3Cl, serosal | 4 | 111.2 ± 5.15 | 36.2 ± 1.6 | 47.2 ± 1.9 |
| La3Cl, serosal | 3 | 110.3 ± 14.9 | 28.3 ± 6.7 | 36.3 ± 7.2 |
| 100 μM amiloride, serosal | 3 | 95.5 ± 2.0 | 24.0 ± 2.4 | 39.0 ± 5.7 |
| Ca2+ | ||||
| No apical Ca2+ | 3 | 28.3 ± 6.1* | 22.6 ± 3.8 | 34.3 ± 4.3 |
| 2-APB | 3 | 54.6 ± 2.6* | 30.6 ± 6.1 | 32.0 ± 6.1 |
| Ryanodine | 3 | 88.3 ± 11.8 | 32.0 ± 3.3 | 44.6 ± 3.3 |
| K+ channels | ||||
| NS1619 | 3 | 82.0 ± 4.6 | 34.6 ± 1.4 | 53.3 ± 3.1 |
| NS309 | 3 | 81.6 ± 12.8 | 44.2 ± 0.3* | 76.0 ± 1.5* |
| Chromakalim | 4 | 94.0 ± 8.1 | 45.7 ± 2.6* | 87.5 ± 3.5* |
| Arachadonic acid | 3 | 95.7 ± 9.0 | 32.3 ± 7.5 | 45.3 ± 5.0 |
| Halothane | 3 | 106.6 ± 0.8 | 35.3 ± 0.8 | 48.5 ± 3.7 |
| Glyburide + chromakalim | 3 | 90.6 ± 5.6 | 32.0 ± 4.5 | 51.0 ± 6.0 |
| Glyburide | 4 | 85.3 ± 12.7 | 26.4 ± 7.2 | 32.6 ± 8.5 |
Tissue was bowed outward at the indicated pressure head, speed, or temperature or after the indicated treatment. Data are mean ± SEM. Cmax was measured at the end of phase 1, Cmin was measured at the end of phase 2, and C60 was measured 60 min after initiation of the mechanical stimulus.
a,b Data are identical and are reproduced for clarity.
* p < 0.05 relative to control.