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. 2009 Jan 1;20(1):21–32. doi: 10.1091/mbc.E08-04-0441

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© 2008 by The American Society for Cell Biology

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Figure 4. — Expression and rescuing activity of VPS-18. (A) VPS-18::GFP expression in embryo (a and b), larva (c and d), adult coelomocyte (e and f), and germline (g and h) as well as the dissected-out germline (i and j). Arrows in (h and j) indicate germline sheath cells. The scale bars represent 20 μm (a–d, i, and j), 5 μm (e and f), and 10 μm (g and h). (B) Rescuing activity of P_vps-18vps-18::gfp, P_vps-18 vps-18(C859A, H861A)::gfp, P_ced-1vps-18 and P_egl-1vps-18 in vps-18(tm1125) embryos. Embryonic cell corpses from 15 animals were scored for comma- (gray bar) and twofold stage (black bar). Comparisons were performed between vps-18(tm1125) and N2 animals and between vps-18(tm1125) and transgenic animals by using unpaired t test. For transgenes, only one transgenic line from a total of three lines that exhibit similar rescuing activity was shown. (C) Rescuing activity of P_vps-18vps-18::gfp, P_vps-18 vps-18(C859A, H861A)::gfp, P_vps-33mVps-18, and P_lim-7vps-18::gfp in vps-18(tm1125) germline. Germ cell corpses in one gonad arm of 15 animals were scored 48 h post-L4 stage for two to three independent transgenic lines as indicated by black, waved, and gray columns. Comparisons were performed between vps-18(tm1125) and all transgenic lines by using unpaired t test. Error bars represent SEM, and double asterisks (**) indicate p < 0.001.