Figure 1.
MEST is a TIF1β primary target gene. (A) Expression of MEST (PEG1), PEG3, PEG10, MEG3, IGF2R, and COPG2 was assessed in TIF1β+/− and TIF1βHP1box/− F9 cells by RT-PCR analysis. HPRT was used as a control house-keeping gene. (B) Schematic representation of the MEST gene with the black boxes representing the exons. The arrow labeled + 1 represents the transcription start site described in Lefebvre et al. (1997). The different regions amplified for the ChIP analysis are shown. (C and D) RA-inducible expression of MEST is independent of TIF1β–HP1 interaction. TIF1β+/− (C) and TIF1βHP1box/− (D) cells were treated for 96 h with either ethanol (no) or 1 μM all-trans RA (RA). MEST expression was measured by qRT-PCR and normalized with HPRT expression. (E) ChIP assay with two anti-TIF1β antibodies (p, PF64 pAb; m, 1TB3 mAb) was performed in WT EC F9 cells. The three positions analyzed on the MEST gene are shown in B, and the HPRT promoter region was also analyzed. Results are the average of at least three independent experiments. *p < 0.05 and **p < 0.005.