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TIF1β-HP1 interaction is permanently required to maintain MEST repression. TIF1βL2/HP1box/CreERT2 cells were treated for the indicated times with 10−7 M OHT and collected for RNA and DNA preparations. (A) Cells were genotyped at each time point by PCR as described previously (Cammas et al., 2004). (B) MEST expression was measured by qRT-PCR at each time and normalized by HPRT expression.
