Figure 1.

Yeast two-hybrid analysis of Rab GTPase binding to distinct coiled-coils in GCC185. (A) Top, secondary structure prediction for GCC185 using Paircoils software. Residue number is shown at bottom. Middle, schematic representation of predicted coiled-coils: CC1, CC2, CC3, and fragments representing the remainder of the protein (ΔCC123, C343, and C110). Bottom, summary of Rab binding to each of the domains as determined by yeast two-hybrid analysis. Rab9A is asterisked because it was variably detected by two hybrid; biochemical experiments shown below confirm its binding to CC3. (B) Yeast two-hybrid data for the construct C343. Each box shows five colonies streaked on selective media; numbers represent activated versions of the human Rab proteins.