Table 5.
Primers used for Sybr green qPCR
| F9 cells | ||
| Gene | Forward | Reverse |
| GAPDH | 5'-GCCAAGAGGGTCATCATCTCC-3' | 5'-TTGGTTCACACCCATCACAAA-3' |
| MVD | 5'-AGCATCGCCCGGCAG-3' | 5'-TGGCCCCTGTAATTTCCCA-3' |
| LSS | 5'-GCGGCTGTGCGATGCT-3' | 5'-AGGTAGCGAACCCGCCA-3' |
| TGFB1 | 5'-TGGAAAGGGCCCAGCAC-3' | 5'-GCAATAGTTGGTATCCAGGGCT-3' |
| IGFII | 5'-AAGAGTTCAGAGAGGCCAAACG-3' | 5'-ATCTCCGAAGAGGCTCCCC-3' |
| MT1 | 5'-TGCTCCACCGGCGG-3' | 5'-TTTGCAGACACAGCCCTGG-3' |
| WNT6 | 5'-GGGCGCTGTCTGAGTCCA-3' | 5'-TGGCCCCTGTAATTTCCCA-3' |
| tPA | 5'-GGCCTGGCACGACACAAT-3' | 5'-CATCACATGGCACCAAGGTC-3' |
| VEGFC | 5'-CAGCTGCGGAAAGGCG-3' | 5'-TTTACACTGTCCCCTGTCCTGG-3' |
| DHODH | 5'-AACACAGGCTACGGGCCAG-3' | 5'-TCCCAGAGGCAGGCCCAT-3' |
| HepG2 cells | ||
| Gene | Forward | Reverse |
| ACADM | 5'-AGCTACCAAGTATGCCCTGGAA-3' | 5'-TAAATGATATTGCTTGGTGCTCTACA-3' |
| ACAT2 | 5'-TGGGCCACCCTCTTGGA-3' | 5'-CCAGTGTGTGTAACAGGGTCACA-3' |
| ACACA | 5'-GCTCCTTGTCACCTGCTTCTG-3' | 5'-TGTAGGCTAGAGATCCCCAAATCA-3' |
| APOA5 | 5'-AGGTGCGCCAGCGACTT-3' | 5'-GCGAGTGAAGGCAGCTATCTG-3' |
| APOC1 | 5'-CAAGGCTCGGGAACTCATCA-3' | 5'-CCCGCATCTTGGCAGAAA-3' |
| APOE | 5'-CGCTGGGTGCAGACACTGT-3' | 5'-AGGCCTTCAACTCCTTCATGGT-3' |
| APOL1 | 5'-TCAGCTGAAAGCGGTGAACA-3' | 5'-CTCTGCTCATTTCCAGGATGCT-3' |
| CYP27A1 | 5'-CCCTGTGGTCCCCACAAA-3' | 5'-GGAAGCCATCAACTTCAATTTCC-3' |
| HMGCR | 5'-CCTGTAACTCAGAGGGTCAAGATGAT-3' | 5'-CCAGCGACTGTGAGCATGAA-3' |
| HMGCS | 5'-TCTTAAATCAAGGCTTGATTCAAGAA-3' | 5'-TGTCCTCTCTGAGCTTCATGTTTT-3' |
| SREBF2 | 5'-CGAATTGAAAGACCTGGTCATG-3' | 5'-TCCTCAGAACGCCAGACTTGT-3' |
| FABP | 5'-CCGCTGGGTCCAAAGTGAT-3' | 5'-CATTGTCTCCAGCTCACATTCC-3' |
| FASN | 5'-GCAAATTCGACCTTTCTCAGAAC-3' | 5'-GGACCCCGTGGAATGTCA-3' |
| LDLR | 5'-AGATAGTGACAATGTCTCACCAAGCT-3' | 5'-CTCACGCTACTGGGCTTCTTCT-3' |
| PPARG | 5'-GCGAAAGCCTTTTGGTGACT-3' | 5'-CAGTGCATTGAACTTCACAGCAA-3' |
| TYMS | 5'-AATCACATCGAGCCACTGAAAA-3' | 5'-AATCCTGAGCTTTGGGAAAGGGT-3' |