. Author manuscript; available in PMC: 2009 Sep 1.

Published in final edited form as: Biochim Biophys Acta. 2008 Jun 30;1782(9):504–510. doi: 10.1016/j.bbadis.2008.05.004

Table I.

Summary of adenosine derivatives with significant effects upon HbF expression.

	Cell count		HbF/HbF±HbA
Name	(x10(6)/ml)		(%)
(Concentration)	EPO	E+S+T	EPO	E+S+T
SQ22536
0 uM	1.3±0.0	1.3±0.1	0.3±0.2	29.5±4.7
30 uM	1.1±0.0	0.7±0.1	0.1±0.0	26.3±7.4
150 uM	0.9±0.0	0.7±0.0	0.3±0.2	12.3±2.2^*
600 uM	0.8±0.0	0.3±0.3	0.2±0.1	2.7±2.6^*

5′-deoxy adenosine
0 uM	1.4±0.5	1.1±0.4	7.1±2.1	49.4±8.2
5 uM	1.0±0.4	1.1±0.5	7.7±2.8	40±1.6
50 uM	0.8±0.4	0.8±0.6	2.8±0.2^*	21.4±4.3
500 uM	0.3±0.3^*	0.3±0.3^*	1.7±0.3^*	NA

2′,3′-dideoxy adenosine
0 uM	1.4±0.5	1.1±0.4	7.1±2.1	49.4±8.2
0.03 uM	1.1±0.7	0.9±0.1	7.1±2.5	44±1.8
0.3 uM	1.0±0.5	0.6±0.1	6.2±3.1	45.5±12
3 uM	1.0±0.5	0.6±0.1^*	4.8±2.2^*	13.1±0.3^*

Asterisk denotes significance of p<0.05 when compared to the controls for each screen. Screens were performed using cells from three separate donors, and adenosine derivatives were added on day 0. Proliferation and HbF production were determined on day 14. Differences in control levels of HbF production were due to the use of separate donor trios for each group of screened molecules.