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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Schizophr Res. 2008 Oct 1;106(2-3):242–247. doi: 10.1016/j.schres.2008.08.028

Figure 1. Validation of the telomerase qPCR assay.

Figure 1

Quantitation using the qPCR-based telomerase assay was evaluated by adding varying amounts of telomere synthetic repeat (TSR) DNA or varying amounts of lymphocyte lysate to the real-time reaction. A. Typical plot depicting relationship between Ct and amount of telomere synthetic repeat (TSR) DNA. To determine if the assay detects telomere DNA in a quantitative manner, increasing amounts of synthetic telomeres (TSR in attomoles) were added to a 25 μL reaction. A linear relationship was observed between Ct and the base 10 logarithm of input telomeres (R2 = 0.995, p<0.0001). B. Typical plot depicting relationship between Ct and the amount of cellular extract derived from lymphocytes. To determine if the assay detects telomerase activity in a quantitative manner, varying amounts of lymphocyte lysate were added to a 25 μL real-time reaction. The units of cellular lysate (ng) refer to the quantity of nucleic acid in the extract, which is proportional to cell number. A linear relationship was observed between Ct and the base 10 logarithm of the amount of lymphocyte lysate (R2 = 0.973, p=0.0019).