Figure 1.

Lot6 is found associated with the yeast 20S proteasome. (A) Analysis of interaction partners of Lot6 (oxidized) by pull-down assays using Lot6–His6 as bait. (B) Purified yeast 20S proteasome (1), purified Lot6–His6 (2), and the first elution fraction of a pull-down assay using Lot6 and purified yeast 20S proteasome (3) were analysed by immunoblotting using both 20S antibody (upper panel) and Lot6 antibody (lower panel). (C) Gel filtration of crude yeast extract. Upper panel, original trace from experiment. Fractions were precipitated and analysed using Lot6 antibody (upper panel) and 20S antibody (lower panel). Std, standard.