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. Author manuscript; available in PMC: 2009 Dec 15.
Published in final edited form as: Int J Cancer. 2008 Dec 15;123(12):2741–2749. doi: 10.1002/ijc.23855

Fig. 2. Increasing amount of wild type and mutant 15-LOX-1 plasmid used in the transfection resulted in increased expression of 15-LOX-1 and phosphorylation of p53.

Fig. 2

HCT-116 cells were transiently transfected with various amounts of expressing plasmid encoding wild type and mutant 15-LOX-1. (A) Western analysis of cell lysates for 15-LOX-1 and p53 phosphorylation. (B) Densitometry analysis of 15-LOX-1 and phosphorylation of p53 as compared with the density of HCT-116 vector cells. Data represents three separate experiments. (C) Western analysis of cell lysates for p53 downstream targets NAG-1 and p21.